Coding
Bm3R1

Part:BBa_K1401000:Design

Designed by: Kathleen Lewis   Group: iGEM14_BostonU   (2014-07-21)


TetR homolog. This is a MoClo part of the Bm3R1 repressor gene with CD fusion sites ('AATG', 'AGGT')


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 200
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 200
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 200
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 200
    Illegal AgeI site found at 540
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

This part originally comes from Bacillus megaterium. The sequence for Bm3R1 came from the Addgene plasmid 49358.

References